PDBe 3up0

X-ray diffraction
1.6Å resolution

Nuclear receptor DAF-12 from hookworm Ancylostoma ceylanicum in complex with (25S)-delta7-dafachronic acid


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
aceDAF-12 Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 27.77 KDa
Source organism: Ancylostoma ceylanicum
Expression system: Escherichia coli BL21(DE3)
  • Canonical: H0USY8 (Residues: 1-243; Coverage: 100%)
Gene name: daf-12
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 14 amino acids
Theoretical weight: 1.71 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: Q15596 (Residues: 740-753; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 48.012Å b: 85.163Å c: 66.127Å
α: 90° β: 107.1° γ: 90°
R R work R free
0.185 0.183 0.202
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided