3unf

X-ray diffraction
2.9Å resolution

Mouse 20S immunoproteasome in complex with PR-957

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Proteasome subunit alpha type-2 Chains: A, O
Molecule details ›
Chains: A, O
Length: 234 amino acids
Theoretical weight: 25.96 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P49722 (Residues: 1-234; Coverage: 100%)
Gene names: Lmpc3, Psma2
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-4 Chains: B, P
Molecule details ›
Chains: B, P
Length: 261 amino acids
Theoretical weight: 29.51 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9R1P0 (Residues: 1-261; Coverage: 100%)
Gene name: Psma4
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-7 Chains: C, Q
Molecule details ›
Chains: C, Q
Length: 248 amino acids
Theoretical weight: 27.9 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9Z2U0 (Residues: 1-248; Coverage: 100%)
Gene name: Psma7
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-5 Chains: D, R
Molecule details ›
Chains: D, R
Length: 241 amino acids
Theoretical weight: 26.44 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9Z2U1 (Residues: 1-241; Coverage: 100%)
Gene name: Psma5
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-1 Chains: E, S
Molecule details ›
Chains: E, S
Length: 263 amino acids
Theoretical weight: 29.59 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9R1P4 (Residues: 1-263; Coverage: 100%)
Gene name: Psma1
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-3 Chains: F, T
Molecule details ›
Chains: F, T
Length: 255 amino acids
Theoretical weight: 28.44 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: O70435 (Residues: 1-255; Coverage: 100%)
Gene name: Psma3
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-6 Chains: G, U
Molecule details ›
Chains: G, U
Length: 246 amino acids
Theoretical weight: 27.41 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9QUM9 (Residues: 1-246; Coverage: 100%)
Gene name: Psma6
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-10 Chains: H, V
Molecule details ›
Chains: H, V
Length: 234 amino acids
Theoretical weight: 24.83 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: O35955 (Residues: 40-273; Coverage: 86%)
Gene names: Lmp10, Mecl1, Psmb10
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-3 Chains: I, W
Molecule details ›
Chains: I, W
Length: 205 amino acids
Theoretical weight: 22.99 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9R1P1 (Residues: 1-205; Coverage: 100%)
Gene name: Psmb3
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-2 Chains: J, X
Molecule details ›
Chains: J, X
Length: 201 amino acids
Theoretical weight: 22.94 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9R1P3 (Residues: 1-201; Coverage: 100%)
Gene name: Psmb2
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-8 Chains: K, Y
Molecule details ›
Chains: K, Y
Length: 204 amino acids
Theoretical weight: 22.67 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P28063 (Residues: 73-276; Coverage: 74%)
Gene names: Lmp7, Mc13, Psmb8
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-1 Chains: L, Z
Molecule details ›
Chains: L, Z
Length: 213 amino acids
Theoretical weight: 23.58 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: O09061 (Residues: 28-240; Coverage: 89%)
Gene name: Psmb1
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-4 Chains: M, a
Molecule details ›
Chains: M, a
Length: 219 amino acids
Theoretical weight: 24.38 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P99026 (Residues: 46-264; Coverage: 83%)
Gene names: Lmp3, Psmb4
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-9 Chains: N, b
Molecule details ›
Chains: N, b
Length: 199 amino acids
Theoretical weight: 21.35 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P28076 (Residues: 21-219; Coverage: 91%)
Gene names: Lmp2, Psmb9, Ring12
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 117.3Å b: 194.6Å c: 157.7Å
α: 90° β: 107.1° γ: 90°
R-values:
R R work R free
0.237 0.235 0.275