3umo

X-ray diffraction
1.7Å resolution

Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Potassium

Released:

Function and Biology Details

Reaction catalysed:
ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase isozyme 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 32.49 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06999 (Residues: 1-309; Coverage: 100%)
Gene names: JW5280, b1723, pfkB
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P2221
Unit cell:
a: 43.812Å b: 88.77Å c: 176.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.208
Expression system: Escherichia coli BL21(DE3)