› 3um3

X-ray diffraction
3.8Å resolution

Crystal structure of the Brox Bro1 domain in complex with the C-terminal tail of CHMP4B

Released:
DOI: 10.2210/pdb3um3/pdb
PDB entry 3um3 coloured by chain, front view.
PDB entry 3um3 coloured by chain, side view.
PDB entry 3um3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Two distinct binding modes define the interaction of Brox with the C-terminal tails of CHMP5 and CHMP4B.
Mu R, Dussupt V, Jiang J, Sette P, Rudd V, Chuenchor W, Bello NF, Bouamr F, Xiao TS
Structure 20 887-98 (2012)
PMID: 22484091

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178444 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
BRO1 domain-containing protein BROX Chain: A
Molecule details ›
Chain: A
Length: 411 amino acids
Theoretical weight: 46.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5VW32 (Residues: 2-411; Coverage: 100%)
Gene names: BROFTI, BROX, C1orf58
Sequence domains: BRO1-like domain
Structure domains: alix/aip1 like domains
Charged multivesicular body protein 4b Chain: B
Molecule details ›
Chain: B
Length: 104 amino acids
Theoretical weight: 11.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H444 (Residues: 121-224; Coverage: 46%)
Gene names: C20orf178, CHMP4B, SHAX1
Sequence domains: Snf7

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6122
Unit cell:
a: 138.156Å b: 138.156Å c: 373.779Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.233 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Membrane fission reactions of the mammalian ESCRT pathway.
McCullough et al. (2013)
2 more

3 mentions without citation

Budding of a Retrovirus: Some Assemblies Required.
Rose et al. (2020)
2 more