3ujg

X-ray diffraction
2.6Å resolution

Crystal structure of SnRK2.6 in complex with HAB1

Released:

Function and Biology Details

Reaction catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase SRK2E Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.05 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q940H6 (Residues: 11-362; Coverage: 97%)
Gene names: At4g33950, F17I5.140, OST1, SNRK2.6, SRK2E
Sequence domains: Protein kinase domain
Structure domains:
Protein phosphatase 2C 16 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.47 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9CAJ0 (Residues: 172-511; Coverage: 70%)
Gene names: At1g72770, F28P22.4, HAB1, P2C-HA
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: I4
Unit cell:
a: 154.835Å b: 154.835Å c: 70.966Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.238
Expression system: Escherichia coli