3ug7

X-ray diffraction
2.9Å resolution

Crystal Structure of Get3 from Methanocaldococcus jannaschii

Released:
Entry authors: Suloway CJM, Rome ME, Clemons Jr WM

Function and Biology Details

Reaction catalysed:
ATP + H(2)O + arsenite(Side 1) = ADP + phosphate + arsenite(Side 2)
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative arsenical pump-driving ATPase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 349 amino acids
Theoretical weight: 39.98 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q58542 (Residues: 1-349; Coverage: 100%)
Gene name: MJ1142
Sequence domains: Anion-transporting ATPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P2
Unit cell:
a: 71.947Å b: 149.636Å c: 72.888Å
α: 90° β: 94.24° γ: 90°
R-values:
R R work R free
0.271 0.269 0.295
Expression system: Escherichia coli BL21(DE3)