› 3ufg

X-ray diffraction
2.55Å resolution

The crystal structure of glycyl-tRNA synthetase subunit alpha from Campylobacter jejuni subsp. jejuni NCTC in complex with ATP

Released:
DOI: 10.2210/pdb3ufg/pdb
PDB 3ufg coloured by chain and viewed from the front.
PDB 3ufg coloured by chain and viewed from the side.
PDB 3ufg coloured by chain and viewed from the top.
Source organism: Campylobacter jejuni
Entry authors: Tan K, Zhou M, Peterson S, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine--tRNA ligase alpha subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 36.25 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9PPK3 (Residues: 1-287; Coverage: 100%)
Gene names: Cj0704, glyQ
Sequence domains: Glycyl-tRNA synthetase alpha subunit
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand LEU 1 x LEU
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R3
Unit cell:
a: 114.58Å b: 114.58Å c: 157.707Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.174 0.255
Expression system: Escherichia coli

Quick links