› 3ufc

X-ray diffraction
2.03Å resolution

Characterization of a Cas6-related gene from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb3ufc/pdb
PDB entry 3ufc coloured by chain, front view.
PDB entry 3ufc coloured by chain, side view.
PDB entry 3ufc coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Crystal structure of a Cas6 paralogous protein from Pyrococcus furiosus.
Park HM, Shin M, Sun J, Kim GS, Lee YC, Park JH, Kim BY, Kim JS
Proteins 80 1895-900 (2012)
PMID: 22447673

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186473 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative CRISPR-associated endoribonuclease-like protein Cas6 Chain: X
Molecule details ›
Chain: X
Length: 243 amino acids
Theoretical weight: 28.2 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U3R3 (Residues: 1-241; Coverage: 100%)
Gene names: PF0393, cas6-2, cas6-3, cas6b
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3121
Unit cell:
a: 79.805Å b: 79.805Å c: 117.362Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.203 0.228
Expression system: Escherichia coli

Quick links

Citations

3 review citations

CRISPR interference: a structural perspective.
Reeks et al. (2013)
2 more

4 mentions without citation

Structural Principles of CRISPR RNA Processing.
Li H. (2015)
3 more