3uez

X-ray diffraction
3.41Å resolution

Crystal structure of the human Colony-Stimulating Factor 1 (hCSF-1) cytokine in complex with the viral receptor BARF1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-136666 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Secreted protein BARF1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 208 amino acids
Theoretical weight: 23.41 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Homo sapiens
UniProt:
  • Canonical: P03228 (Residues: 21-221; Coverage: 100%)
Gene name: BARF1
Structure domains: Immunoglobulins
Macrophage colony-stimulating factor 1 43 kDa subunit Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 153 amino acids
Theoretical weight: 17.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09603 (Residues: 33-181; Coverage: 29%)
Gene name: CSF1
Sequence domains: Macrophage colony stimulating factor-1 (CSF-1)
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: R3
Unit cell:
a: 235.24Å b: 235.24Å c: 95.71Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.231 0.274
Expression systems:
  • Homo sapiens
  • Escherichia coli