› 3ubm

X-ray diffraction
1.99Å resolution

Formyl-CoA:oxalate CoA-transferase from Acetobacter aceti

Released:
DOI: 10.2210/pdb3ubm/pdb
PDB entry 3ubm coloured by chain, front view.
PDB entry 3ubm coloured by chain, side view.
PDB entry 3ubm coloured by chain, top view.
Source organism: Acetobacter aceti
Primary publication:
Formyl-coenzyme A (CoA):oxalate CoA-transferase from the acidophile Acetobacter aceti has a distinctive electrostatic surface and inherent acid stability.
Mullins EA, Starks CM, Francois JA, Sael L, Kihara D, Kappock TJ
Protein Sci 21 686-96 (2012)
PMID: 22374910

Function and Biology Details

Reaction catalysed: 
Formyl-CoA + oxalate = formate + oxalyl-CoA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109081 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formyl-CoA:oxalate CoA-transferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 456 amino acids
Theoretical weight: 49.8 KDa
Source organism: Acetobacter aceti
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A9X6P7 (Residues: 1-436; Coverage: 100%)
Gene name: frc
Sequence domains: CoA-transferase family III
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand COA 4 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 98.164Å b: 121.612Å c: 161.377Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.242
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Mechanisms of acid tolerance in bacteria and prospects in biotechnology and bioremediation.
Liu et al. (2015)
1 more

5 mentions without citation

Formyl-coenzyme A (CoA):oxalate CoA-transferase from the acidophile Acetobacter aceti has a distinctive electrostatic surface and inherent acid stability.
Mullins et al. (2012)
4 more