3ub0

X-ray diffraction
2.6Å resolution

Crystal structure of the nonstructural protein 7 and 8 complex of Feline Coronavirus

Released:

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Non-structural protein 8 Chains: A, D
Molecule details ›
Chains: A, D
Length: 199 amino acids
Theoretical weight: 22.02 KDa
Source organism: Feline infectious peritonitis virus (strain 79-1146)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q98VG9 (Residues: 3583-3777; Coverage: 3%)
Gene names: 1a-1b, rep
Sequence domains: Coronavirus replicase NSP8
Structure domains:
Non-structural protein 7 Chains: B, C, E, F
Molecule details ›
Chains: B, C, E, F
Length: 87 amino acids
Theoretical weight: 9.75 KDa
Source organism: Feline infectious peritonitis virus (strain 79-1146)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q98VG9 (Residues: 3500-3582; Coverage: 1%)
Gene names: 1a-1b, rep
Sequence domains: Coronavirus replicase NSP7

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2221
Unit cell:
a: 121.454Å b: 160.301Å c: 102.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.227
Expression system: Escherichia coli