3uaw

X-ray diffraction
1.2Å resolution

Crystal structure of adenosine phosphorylase from Bacillus cereus complexed with adenosine

Released:
Source organism: Bacillus cereus
Primary publication:
Structural basis of the substrate specificity of Bacillus cereus adenosine phosphorylase.
Acta Crystallogr. D Biol. Crystallogr. 68 239-48 (2012)
PMID: 22349225

Function and Biology Details

Reaction catalysed:
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase DeoD-type Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 25.7 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5EEL8 (Residues: 1-235; Coverage: 100%)
Gene name: deoD
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6322
Unit cell:
a: 121Å b: 121Å c: 67.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.126 0.125 0.141
Expression system: Escherichia coli BL21(DE3)