X-ray diffraction
1.4Å resolution

Crystal structure of adenosine phosphorylase from Bacillus cereus

Source organism: Bacillus cereus
Primary publication:
Structural basis of the substrate specificity of Bacillus cereus adenosine phosphorylase.
Acta Crystallogr. D Biol. Crystallogr. 68 239-48 (2012)
PMID: 22349225

Function and Biology Details

Reaction catalysed:
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Purine nucleoside phosphorylase DeoD-type Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 25.7 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q5EEL8 (Residues: 1-235; Coverage: 100%)
Gene name: deoD
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6322
Unit cell:
a: 122Å b: 122Å c: 68Å
α: 90° β: 90° γ: 120°
R R work R free
0.15 0.149 0.164
Expression system: Escherichia coli BL21(DE3)