› 3u9z

X-ray diffraction
2.09Å resolution

Crystal structure between actin and a protein construct containing the first beta-thymosin domain of drosophila ciboulot (residues 2-58) with the three mutations N26D/Q27K/D28S

Released:
DOI: 10.2210/pdb3u9z/pdb
PDB entry 3u9z coloured by chain, front view.
PDB entry 3u9z coloured by chain, side view.
PDB entry 3u9z coloured by chain, top view.
Source organisms:
Primary publication:
How a single residue in individual β-thymosin/WH2 domains controls their functions in actin assembly.
Didry D, Cantrelle FX, Husson C, Roblin P, Moorthy AM, Perez J, Le Clainche C, Hertzog M, Guittet E, Carlier MF, van Heijenoort C, Renault L
EMBO J 31 1000-13 (2012)
PMID: 22193718

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132189 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Ciboulot, isoform D Chain: C
Molecule details ›
Chain: C
Length: 58 amino acids
Theoretical weight: 6.26 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O97428 (Residues: 2-58; Coverage: 44%)
Gene names: CG4944, Dmel\CG4944, Dmel_CG4944, EG0007-11, EG:EG0007.11, cib
Sequence domains: Thymosin beta-4 family

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 45.795Å b: 75.181Å c: 117.708Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.16 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Disordered proteinaceous machines.
Fuxreiter et al. (2014)
9 more

1 mention without citation

Classification of intrinsically disordered regions and proteins.
van der Lee et al. (2014)