X-ray diffraction
3.03Å resolution

Crystal structure of extracellular region of human epidermal growth factor receptor 4 in complex with neuregulin-1 beta

Source organism: Homo sapiens
Primary publication:
A single ligand is sufficient to activate EGFR dimers.
Proc. Natl. Acad. Sci. U.S.A. 109 10861-6 (2012)
PMID: 22699492

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Receptor tyrosine-protein kinase erbB-4 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 615 amino acids
Theoretical weight: 68.74 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
  • Canonical: Q15303 (Residues: 26-640; Coverage: 48%)
Gene names: ERBB4, HER4
Sequence domains:
Structure domains:
Neuregulin-1 Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 55 amino acids
Theoretical weight: 6.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q02297 (Residues: 175-225; Coverage: 8%)
  • Best match: Q02297-12 (Residues: 175-229)
Gene names: GGF, HGL, HRGA, NDF, NRG1, SMDF
Sequence domains: EGF-like domain
Structure domains: Laminin

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 85.73Å b: 223.51Å c: 146.92Å
α: 90° β: 99.72° γ: 90°
R R work R free
0.191 0.19 0.227
Expression systems:
  • Cricetulus griseus
  • Escherichia coli