3u49

X-ray diffraction
1.75Å resolution

Crystal structure of YwfH, NADPH dependent reductase involved in Bacilysin biosynthesis

Released:
Source organism: Bacillus subtilis
Primary publication:
Structural insights into the role of Bacillus subtilis YwfH (BacG) in tetrahydrotyrosine synthesis.
Acta Crystallogr. D Biol. Crystallogr. 69 324-32 (2013)
PMID: 23519407

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH-dependent reductase BacG Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 281 amino acids
Theoretical weight: 30.47 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P39644 (Residues: 1-259; Coverage: 100%)
Gene names: BSU37680, bacG, ipa-86r, ywfH
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 64.85Å b: 136.99Å c: 66.7Å
α: 90° β: 117.99° γ: 90°
R-values:
R R work R free
0.19 0.189 0.222
Expression system: Escherichia coli