3u3z

X-ray diffraction
1.5Å resolution

Structure of human microcephalin (MCPH1) tandem BRCT domains in complex with an H2A.X peptide phosphorylated at Ser139 and Tyr142

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-147616 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Microcephalin Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 21.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8NEM0 (Residues: 640-835; Coverage: 24%)
Gene name: MCPH1
Structure domains: BRCT domain
Histone H2AX Chain: B
Molecule details ›
Chain: B
Length: 4 amino acids
Theoretical weight: 685 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P16104 (Residues: 140-143; Coverage: 3%)
Gene names: H2AFX, H2AX

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 37.689Å b: 46.537Å c: 55.778Å
α: 90° β: 97.07° γ: 90°
R-values:
R R work R free
0.129 0.129 0.171
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided