› 3u0g

X-ray diffraction
1.9Å resolution

Crystal structure of branched-chain amino acid aminotransferase from burkholderia pseudomallei

Released:
DOI: 10.2210/pdb3u0g/pdb
PDB entry 3u0g coloured by chain, front view.
PDB entry 3u0g coloured by chain, side view.
PDB entry 3u0g coloured by chain, top view.
Source organism: Burkholderia pseudomallei
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Branched-chain-amino-acid aminotransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 328 amino acids
Theoretical weight: 36.32 KDa
Source organism: Burkholderia pseudomallei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3JVJ9 (Residues: 1-307; Coverage: 100%)
Gene names: BURPS1710b_0992, ilvE
Sequence domains: Amino-transferase class IV
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PO4 6 x PO4
Ligand CL 6 x CL
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: C2221
Unit cell:
a: 106.97Å b: 139.27Å c: 290.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.23
Expression system: Escherichia coli BL21(DE3)

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