3tx9

X-ray diffraction
2Å resolution

OYE1 complexed with 2-(Hydroxymethyl)-cyclopent-2-enone

Released:
Source organism: Saccharomyces pastorianus
Entry authors: Pompeu YA, Stewart JD

Function and Biology Details

Reaction catalysed:
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH dehydrogenase 1 Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 45.07 KDa
Source organism: Saccharomyces pastorianus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02899 (Residues: 1-400; Coverage: 100%)
Gene name: OYE1
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P43212
Unit cell:
a: 141.188Å b: 141.188Å c: 42.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.182 0.216
Expression system: Escherichia coli BL21(DE3)