› 3tqq

X-ray diffraction
2Å resolution

Structure of the methionyl-tRNA formyltransferase (fmt) from Coxiella burnetii

Released:
DOI: 10.2210/pdb3tqq/pdb
PDB entry 3tqq coloured by chain, front view.
PDB entry 3tqq coloured by chain, side view.
PDB entry 3tqq coloured by chain, top view.
Source organism: Coxiella burnetii
Primary publication:
Structural genomics for drug design against the pathogen Coxiella burnetii.
Franklin MC, Cheung J, Rudolph MJ, Burshteyn F, Cassidy M, Gary E, Hillerich B, Yao ZK, Carlier PR, Totrov M, Love JD
Proteins 83 2124-36 (2015)
PMID: 26033498

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182893 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionyl-tRNA formyltransferase Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 34.66 KDa
Source organism: Coxiella burnetii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83AA8 (Residues: 1-314; Coverage: 100%)
Gene names: CBU_1997, fmt
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand K 1 x K
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 51.82Å b: 56.295Å c: 62.868Å
α: 90° β: 113.66° γ: 90°
R-values:
R R work R free
0.198 0.196 0.246
Expression system: Escherichia coli

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Citations

4 review citations

Laccases of prokaryotic origin: enzymes at the interface of protein science and protein technology.
Martins et al. (2015)
3 more