PDBe 3tq7

X-ray diffraction
2.3Å resolution

EB1c/EB3c heterodimer in complex with the CAP-Gly domain of P150glued

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Microtubule-associated protein RP/EB family member 1 Chain: A
Molecule details ›
Chain: A
Length: 78 amino acids
Theoretical weight: 9.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15691 (Residues: 191-268; Coverage: 29%)
Gene name: MAPRE1
Sequence domains: EB1-like C-terminal motif
Microtubule-associated protein RP/EB family member 3 Chain: B
Molecule details ›
Chain: B
Length: 82 amino acids
Theoretical weight: 9.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UPY8 (Residues: 200-281; Coverage: 29%)
Gene name: MAPRE3
Sequence domains: EB1-like C-terminal motif
Dynactin subunit 1 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 71 amino acids
Theoretical weight: 7.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14203 (Residues: 27-97; Coverage: 6%)
Gene name: DCTN1
Sequence domains: CAP-Gly domain
Structure domains: CAP Gly-rich-like domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 41.15Å b: 103.56Å c: 48.13Å
α: 90° β: 113.64° γ: 90°
R-values:
R R work R free
0.229 0.226 0.281
Expression system: Escherichia coli