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Human DNA Polymerase eta in binary complex with DNA

X-ray diffraction
2.556Å resolution
PDB entry 3tq1 coloured by chain, front view.
PDB entry 3tq1 coloured by chain, side view.
PDB entry 3tq1 coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA polymerase eta and DNA
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Multimeric state: hetero trimer
Accessible surface area: 21605.53 Å2
Buried surface area: 3690.85 Å2
Dissociation area: 922.58 Å2
Dissociation energy (ΔGdiss): 21.36 kcal/mol
Dissociation entropy (TΔSdiss): 9.32 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-116941

Macromolecules

DNA polymerase eta
Chain: A
Length: 435 amino acids
Theoretical weight: 48.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y253 (Residues: 1-432; Coverage: 61%)
Gene names: POLH, RAD30, RAD30A, XPV
Pfam:
InterPro:
CATH:
DNA polymerase eta in PDB entry 3tq1, assembly 1, front view.
DNA polymerase eta in PDB entry 3tq1, assembly 1, side view.
DNA polymerase eta in PDB entry 3tq1, assembly 1, top view.
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DNA chain T
Name: DNA (5'-D(*TP*CP*AP*TP*TP*AP*TP*GP*AP*CP*GP*CP*T)-3')
Representative chains: T
Source organism: Homo sapiens [9606]
Expression system: Not provided
Length: 13 nucleotides
Theoretical weight: 3.94 KDa
DNA (5'-D(*TP*CP*AP*TP*TP*AP*TP*GP*AP*CP*GP*CP*T)-3') in PDB entry 3tq1, assembly 1, front view.
DNA (5'-D(*TP*CP*AP*TP*TP*AP*TP*GP*AP*CP*GP*CP*T)-3') in PDB entry 3tq1, assembly 1, side view.
DNA (5'-D(*TP*CP*AP*TP*TP*AP*TP*GP*AP*CP*GP*CP*T)-3') in PDB entry 3tq1, assembly 1, top view.
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DNA chain P
Name: DNA (5'-D(*TP*AP*GP*CP*GP*TP*CP*AP*T)-3')
Representative chains: P
Source organism: Homo sapiens [9606]
Expression system: Not provided
Length: 9 nucleotides
Theoretical weight: 2.73 KDa
DNA (5'-D(*TP*AP*GP*CP*GP*TP*CP*AP*T)-3') in PDB entry 3tq1, assembly 1, front view.
DNA (5'-D(*TP*AP*GP*CP*GP*TP*CP*AP*T)-3') in PDB entry 3tq1, assembly 1, side view.
DNA (5'-D(*TP*AP*GP*CP*GP*TP*CP*AP*T)-3') in PDB entry 3tq1, assembly 1, top view.
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