› 3tnm

X-ray diffraction
1.85Å resolution

Crystal structure of A32 Fab, an ADCC mediating anti-HIV-1 antibody

Released:
DOI: 10.2210/pdb3tnm/pdb
PDB 3tnm coloured by chain and viewed from the front.
PDB 3tnm coloured by chain and viewed from the side.
PDB 3tnm coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Diverse specificity and effector function among human antibodies to HIV-1 envelope glycoprotein epitopes exposed by CD4 binding.
Guan Y, Pazgier M, Sajadi MM, Kamin-Lewis R, Al-Darmarki S, Flinko R, Lovo E, Wu X, Robinson JE, Seaman MS, Fouts TR, Gallo RC, DeVico AL, Lewis GK
Proc. Natl. Acad. Sci. U.S.A. 110 E69-78 (2013)
PMID: 23237851

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fab heavy chain of human anti-HIV-1 Env antibody A32 Chains: A, H
Molecule details ›
Chains: A, H
Length: 231 amino acids
Theoretical weight: 24.77 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Fab light chain of human anti-HIV-1 Env antibody A32 Chains: B, L
Molecule details ›
Chains: B, L
Length: 216 amino acids
Theoretical weight: 22.83 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P1
Unit cell:
a: 40.78Å b: 71.316Å c: 77.673Å
α: 103.69° β: 104.02° γ: 105.4°
R-values:
R R work R free
0.19 0.187 0.232
Expression system: Homo sapiens

Quick links

Citations

23 review citations

Building a better antibody through the Fc: advances and challenges in harnessing antibody Fc effector functions for antiviral protection.
Gunn et al. (2021)
22 more

8 mentions without citation

Diverse specificity and effector function among human antibodies to HIV-1 envelope glycoprotein epitopes exposed by CD4 binding.
Guan et al. (2013)
7 more