PDBe 3tma

X-ray diffraction
2.05Å resolution

Crystal structure of TrmN from Thermus thermophilus

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + guanine(6) in tRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(6) in tRNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (guanine(6)-N2)-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 39.08 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72IH5 (Residues: 1-335; Coverage: 100%)
Gene names: TT_C1157, trmN
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P3221
Unit cell:
a: 65.86Å b: 65.86Å c: 144.55Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.154 0.212
Expression system: Escherichia coli BL21(DE3)