PDBe 3tm4

X-ray diffraction
1.95Å resolution

Crystal structure of Trm14 from Pyrococcus furiosus in complex with S-adenosylmethionine


Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + guanine(6) in tRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(6) in tRNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
tRNA (guanine(6)-N2)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 373 amino acids
Theoretical weight: 42.13 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
  • Canonical: Q8U248 (Residues: 1-365; Coverage: 100%)
Gene names: PF1002, trm14
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 82.08Å b: 45.17Å c: 121.43Å
α: 90° β: 91.79° γ: 90°
R R work R free
0.183 0.182 0.196
Expression system: Escherichia coli