› 3tle

X-ray diffraction
1.3Å resolution

Microcin C7 self immunity protein MccF in complex with glutamyl sulfamoyl adenylate

Released:
DOI: 10.2210/pdb3tle/pdb
PDB entry 3tle coloured by chain, front view.
PDB entry 3tle coloured by chain, side view.
PDB entry 3tle coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic microcin C7.
Agarwal V, Tikhonov A, Metlitskaya A, Severinov K, Nair SK
Proc Natl Acad Sci U S A 109 4425-30 (2012)
PMID: 22388748

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175250 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Microcin C7 self-immunity protein MccF Chains: A, B
Molecule details ›
Chains: A, B
Length: 371 amino acids
Theoretical weight: 41.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47511 (Residues: 2-344; Coverage: 100%)
Gene name: mccF
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GSU 2 x GSU
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 54.359Å b: 85.81Å c: 73.006Å
α: 90° β: 101.26° γ: 90°
R-values:
R R work R free
0.179 0.178 0.197
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Microcins in Enterobacteriaceae: Peptide Antimicrobials in the Eco-Active Intestinal Chemosphere.
Baquero et al. (2019)
3 more

1 mention without citation

Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic microcin C7.
Agarwal et al. (2012)