› 3tl4

X-ray diffraction
2.3Å resolution

Crystal Structure of the tRNA Binding Domain of Glutaminyl-tRNA Synthetase from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb3tl4/pdb
PDB entry 3tl4 coloured by chain, front view.
PDB entry 3tl4 coloured by chain, side view.
PDB entry 3tl4 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural conservation of an ancient tRNA sensor in eukaryotic glutaminyl-tRNA synthetase.
Grant TD, Snell EH, Luft JR, Quartley E, Corretore S, Wolfley JR, Snell ME, Hadd A, Perona JJ, Phizicky EM, Grayhack EJ
Nucleic Acids Res 40 3723-31 (2012)
PMID: 22180531

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine--tRNA ligase Chain: X
Molecule details ›
Chain: X
Length: 187 amino acids
Theoretical weight: 21.5 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P13188 (Residues: 1-187; Coverage: 23%)
Gene names: GLN4, O3601, YOR168W
Sequence domains: Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 40.8Å b: 34.62Å c: 74.269Å
α: 90° β: 97.61° γ: 90°
R-values:
R R work R free
0.184 0.182 0.211
Expression system: Saccharomyces cerevisiae

Quick links

Citations

1 review citation

Architecture and metamorphosis.
Guo et al. (2014)
9 other articles

4 mentions without citation

Asc1, homolog of human RACK1, prevents frameshifting in yeast by ribosomes stalled at CGA codon repeats.
Wolf et al. (2015)
3 more