3thy

X-ray diffraction
2.89Å resolution

Human MutSbeta complexed with an IDL of 2 bases (Loop2) and ADP

Released:
Source organism: Homo sapiens
Primary publication:
Mechanism of mismatch recognition revealed by human MutSĪ² bound to unpaired DNA loops.
Nat. Struct. Mol. Biol. 19 72-8 (2012)
PMID: 22179786

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA mismatch repair protein Msh2 Chain: A
Molecule details ›
Chain: A
Length: 934 amino acids
Theoretical weight: 104.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43246 (Residues: 1-934; Coverage: 100%)
Gene name: MSH2
Sequence domains:
Structure domains:
DNA mismatch repair protein Msh3 Chain: B
Molecule details ›
Chain: B
Length: 918 amino acids
Theoretical weight: 104.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P20585 (Residues: 219-1134; Coverage: 81%)
Gene names: DUC1, DUG, MSH3
Sequence domains:
Structure domains:
DNA Loop2 minus strand Chain: D
Molecule details ›
Chain: D
Length: 24 nucleotides
Theoretical weight: 7.35 KDa
DNA Loop2 plus strand Chain: E
Molecule details ›
Chain: E
Length: 24 nucleotides
Theoretical weight: 7.37 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P1
Unit cell:
a: 66.27Å b: 91.106Å c: 95.628Å
α: 67.82° β: 86.98° γ: 73.35°
R-values:
R R work R free
0.196 0.194 0.274
Expression system: Spodoptera frugiperda