› 3t9w

X-ray diffraction
1.5Å resolution

Small laccase from Amycolatopsis sp. ATCC 39116

Released:
DOI: 10.2210/pdb3t9w/pdb
PDB entry 3t9w coloured by chain, front view.
PDB entry 3t9w coloured by chain, side view.
PDB entry 3t9w coloured by chain, top view.
Source organism: Amycolatopsis sp. ATCC 39116
Primary publication:
Roles of small laccases from Streptomyces in lignin degradation.
Majumdar S, Lukk T, Solbiati JO, Bauer S, Nair SK, Cronan JE, Gerlt JA
Biochemistry 53 4047-58 (2014)
PMID: 24870309

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small laccase, multi-copper oxidase Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 33.25 KDa
Source organism: Amycolatopsis sp. ATCC 39116
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: J9PBQ8 (Residues: 1-279; Coverage: 100%)
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
Ligand NI 1 x NI
Ligand CU 4 x CU
Ligand PEO 1 x PEO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: R32
Unit cell:
a: 110.18Å b: 110.18Å c: 178.82Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.172 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Lignocellulose degradation mechanisms across the Tree of Life.
Cragg et al. (2015)
18 more

3 mentions without citation

Effect of the L499M mutation of the ascomycetous Botrytis aclada laccase on redox potential and catalytic properties.
Osipov et al. (2014)
2 more