X-ray diffraction
2.5Å resolution

Crystal structure of ketosteroid isomerase Y14AY55FD99A from Pseudomonas testosteroni


Function and Biology Details

Reaction catalysed:
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Steroid Delta-isomerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 125 amino acids
Theoretical weight: 13.26 KDa
Source organism: Comamonas testosteroni
Expression system: Escherichia coli BL21
  • Canonical: P00947 (Residues: 1-125; Coverage: 100%)
Gene name: ksi
Sequence domains: Nuclear transport factor 2 (NTF2) domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P6122
Unit cell:
a: 64.227Å b: 64.227Å c: 496.554Å
α: 90° β: 90° γ: 120°
R R work R free
0.242 0.239 0.3
Expression system: Escherichia coli BL21