› 3t79

X-ray diffraction
3.61Å resolution

Ndc10: a platform for inner kinetochore assembly in budding yeast

Released:
DOI: 10.2210/pdb3t79/pdb
PDB 3t79 coloured by chain and viewed from the front.
PDB 3t79 coloured by chain and viewed from the side.
PDB 3t79 coloured by chain and viewed from the top.
Source organism: Kluyveromyces lactis NRRL Y-1140
Primary publication:
Ndc10 is a platform for inner kinetochore assembly in budding yeast.
Cho US, Harrison SC
Nat. Struct. Mol. Biol. 19 48-55 (2012)
PMID: 22139014

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
hetero trimer
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
CCR4-NOT transcription complex subunit 11 Chains: A, D
Molecule details ›
Chains: A, D
Length: 402 amino acids
Theoretical weight: 46.61 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CPM4 (Residues: 1-402; Coverage: 55%)
Gene name: KLLA0_E03807g
Sequence domains: Centromere DNA-binding protein complex CBF3 subunit, domain 2
Structure domains:
DNA (5'-D(P*TP*TP*AP*AP*TP*TP*TP*AP*TP*AP*AP*AP*AP*TP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Not provided
DNA (5'-D(P*AP*AP*AP*TP*TP*TP*TP*AP*TP*AP*AP*AP*TP*TP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Not provided
DNA (5'-D(P*TP*AP*AP*TP*TP*TP*AP*TP*AP*AP*AP*AP*TP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Not provided
DNA (5'-D(P*AP*AP*AP*AP*AP*TP*TP*TP*TP*AP*TP*AP*AP*AP*T)-3') Chain: F
Molecule details ›
Chain: F
Length: 15 nucleotides
Theoretical weight: 4.6 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 93.06Å b: 99.651Å c: 125.915Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.288 0.286 0.331
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Establishing correct kinetochore-microtubule attachments in mitosis and meiosis.
Cairo et al. (2020)
8 more

1 mention without citation

Ndc10 is a platform for inner kinetochore assembly in budding yeast.
Cho et al. (2011)