3t79

X-ray diffraction
3.61Å resolution

Ndc10: a platform for inner kinetochore assembly in budding yeast

Released:
Primary publication:
Ndc10 is a platform for inner kinetochore assembly in budding yeast.
Nat. Struct. Mol. Biol. 19 48-55 (2012)
PMID: 22139014

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
hetero trimer
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
CCR4-NOT transcription complex subunit 11 Chains: A, D
Molecule details ›
Chains: A, D
Length: 402 amino acids
Theoretical weight: 46.61 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CPM4 (Residues: 1-402; Coverage: 55%)
Gene name: KLLA0_E03807g
Sequence domains: Centromere DNA-binding protein complex CBF3 subunit, domain 2
Structure domains:
DNA (5'-D(P*TP*TP*AP*AP*TP*TP*TP*AP*TP*AP*AP*AP*AP*TP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Not provided
DNA (5'-D(P*AP*AP*AP*TP*TP*TP*TP*AP*TP*AP*AP*AP*TP*TP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Not provided
DNA (5'-D(P*TP*AP*AP*TP*TP*TP*AP*TP*AP*AP*AP*AP*TP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Not provided
DNA (5'-D(P*AP*AP*AP*AP*AP*TP*TP*TP*TP*AP*TP*AP*AP*AP*T)-3') Chain: F
Molecule details ›
Chain: F
Length: 15 nucleotides
Theoretical weight: 4.6 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 93.06Å b: 99.651Å c: 125.915Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.288 0.286 0.331
Expression systems:
  • Escherichia coli
  • Not provided