3t6c

X-ray diffraction
1.6Å resolution

Crystal structure of an enolase from pantoea ananatis (efi target efi-501676) with bound d-gluconate and mg

Released:
Source organism: Pantoea ananatis LMG 20103
Entry authors: Vetting MW, Toro R, Bhosle R, Wasserman SR, Morisco LL, Hillerich B, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactonate dehydratase family member RspA Chains: A, B
Molecule details ›
Chains: A, B
Length: 440 amino acids
Theoretical weight: 48.87 KDa
Source organism: Pantoea ananatis LMG 20103
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D4GJ14 (Residues: 1-417; Coverage: 100%)
Gene names: PANA_0592, rspA
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P4212
Unit cell:
a: 130.79Å b: 130.79Å c: 104.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.139 0.168
Expression system: Escherichia coli BL21(DE3)