› 3t61

X-ray diffraction
2.2Å resolution

Crystal Structure of a gluconokinase from Sinorhizobium meliloti 1021

Released:
DOI: 10.2210/pdb3t61/pdb
PDB entry 3t61 coloured by chain, front view.
PDB entry 3t61 coloured by chain, side view.
PDB entry 3t61 coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Kumaran D, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, LaFleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
ATP + D-gluconate = ADP + 6-phospho-D-gluconate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gluconokinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 22.65 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92VK3 (Residues: 1-178; Coverage: 100%)
Gene names: SM_b21119, gntK
Sequence domains: AAA domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P43
Unit cell:
a: 68.202Å b: 68.202Å c: 118.621Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.25
Expression system: Escherichia coli BL21(DE3)

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