› 3t5v

X-ray diffraction
2.9Å resolution

Sac3:Thp1:Sem1 complex

Released:
DOI: 10.2210/pdb3t5v/pdb
PDB entry 3t5v coloured by chain, front view.
PDB entry 3t5v coloured by chain, side view.
PDB entry 3t5v coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex.
Ellisdon AM, Dimitrova L, Hurt E, Stewart M
Nat Struct Mol Biol 19 328-36 (2012)
PMID: 22343721

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-131730 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nuclear mRNA export protein SAC3 Chains: A, D
Molecule details ›
Chains: A, D
Length: 316 amino acids
Theoretical weight: 36.94 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P46674 (Residues: 250-563; Coverage: 24%)
Gene names: LEP1, SAC3, YD8358.13, YDR159W
Sequence domains: SAC3/GANP family
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Nuclear mRNA export protein THP1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 455 amino acids
Theoretical weight: 52.73 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08231 (Residues: 1-455; Coverage: 100%)
Gene names: BUD29, O1140, THP1, YOL072W
Sequence domains: PCI domain
26S proteasome complex subunit SEM1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 89 amino acids
Theoretical weight: 10.4 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: O94742 (Residues: 1-89; Coverage: 100%)
Gene names: DSH1, SEM1, YDR363W-A
Sequence domains: DSS1/SEM1 family
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P4122
Unit cell:
a: 164.77Å b: 164.77Å c: 276.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.231
Expression system: Escherichia coli

Quick links

Citations

14 review citations

RNA in cancer.
Goodall et al. (2021)
13 more

9 mentions without citation

Structural biology of the PCI-protein fold.
Ellisdon et al. (2012)
8 more