› 3t5s

X-ray diffraction
2.3Å resolution

Structure of macrophage migration inhibitory factor from Giardia lamblia

Released:
DOI: 10.2210/pdb3t5s/pdb
PDB entry 3t5s coloured by chain, front view.
PDB entry 3t5s coloured by chain, side view.
PDB entry 3t5s coloured by chain, top view.
Source organism: Giardia lamblia ATCC 50803
Primary publication:
Crystal structure of a macrophage migration inhibitory factor from Giardia lamblia.
Buchko GW, Abendroth J, Robinson H, Zhang Y, Hewitt SN, Edwards TE, Van Voorhis WC, Myler PJ
J Struct Funct Genomics 14 47-57 (2013)
PMID: 23709284

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-dopachrome isomerase Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 14.67 KDa
Source organism: Giardia lamblia ATCC 50803
Expression system: Escherichia coli
UniProt:
  • Canonical: A8BFP4 (Residues: 1-114; Coverage: 100%)
Gene names: GL50803_0012091, GL50803_12091
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A, APS BEAMLINE 23-ID-D
Spacegroup: I213
Unit cell:
a: 95.55Å b: 95.55Å c: 95.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.268
Expression system: Escherichia coli

Quick links

Citations

3 review citations

MIF, a controversial cytokine: a review of structural features, challenges, and opportunities for drug development.
Bloom et al. (2016)
2 more

1 mention without citation

Crystal structure of a macrophage migration inhibitory factor from Giardia lamblia.
Buchko et al. (2013)