3t5g

X-ray diffraction
1.7Å resolution

Structure of fully modified farnesylated Rheb in complex with PDE6D

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTP-binding protein Rheb Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 20.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15382 (Residues: 1-181; Coverage: 98%)
Gene names: RHEB, RHEB2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta Chain: B
Molecule details ›
Chain: B
Length: 152 amino acids
Theoretical weight: 17.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43924 (Residues: 1-150; Coverage: 100%)
Gene names: PDE6D, PDED
Sequence domains: GMP-PDE, delta subunit
Structure domains: GMP phosphodiesterase, delta subunit

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 47.89Å b: 57.38Å c: 134.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.235
Expression system: Escherichia coli