3t4c

X-ray diffraction
1.95Å resolution

Function and Biology Details

Reaction catalysed:
Phosphoenolpyruvate + D-arabinose 5-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydro-3-deoxyphosphooctonate aldolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 288 amino acids
Theoretical weight: 31.27 KDa
Source organism: Burkholderia ambifaria MC40-6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1YT10 (Residues: 1-284; Coverage: 100%)
Gene names: BamMC406_2018, kdsA
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 49.13Å b: 129.5Å c: 169.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.203
Expression system: Escherichia coli BL21(DE3)