3t0e

X-ray diffraction
4Å resolution

Crystal structure of a complete ternary complex of T cell receptor, peptide-MHC and CD4

Released:
Source organism: Homo sapiens
Primary publication:
Crystal structure of a complete ternary complex of T-cell receptor, peptide-MHC, and CD4.
Proc. Natl. Acad. Sci. U.S.A. 109 5405-10 (2012)
PMID: 22431638

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 21.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01903 (Residues: 26-207; Coverage: 80%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1 beta chain Chain: B
Molecule details ›
Chain: B
Length: 221 amino acids
Theoretical weight: 25.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01911 (Residues: 30-221; Coverage: 81%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:
T cell receptor alpha chain constant Chain: C
Molecule details ›
Chain: C
Length: 206 amino acids
Theoretical weight: 22.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 1-94; Coverage: 67%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T cell receptor beta constant 2 Chain: D
Molecule details ›
Chain: D
Length: 245 amino acids
Theoretical weight: 27.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A5B9 (Residues: 1-130; Coverage: 73%)
Gene names: TCRBC2, TRBC2
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T-cell surface glycoprotein CD4 Chain: E
Molecule details ›
Chain: E
Length: 373 amino acids
Theoretical weight: 41.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P01730 (Residues: 26-388; Coverage: 84%)
Gene name: CD4
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P43212
Unit cell:
a: 146.151Å b: 146.151Å c: 231.436Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.238 0.305
Expression systems:
  • Escherichia coli BL21(DE3)
  • Spodoptera frugiperda