PDBe 3szm

X-ray diffraction
2.63Å resolution

STRUCTURE OF HUMAN MICROCEPHALIN (MCPH1) TANDEM BRCT DOMAINS IN COMPLEX WITH A GAMMA-H2AX PHOSPHOPEPTIDE

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Microcephalin Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 199 amino acids
Theoretical weight: 21.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8NEM0 (Residues: 640-835; Coverage: 24%)
Gene name: MCPH1
Sequence domains: BRCT domain, a BRCA1 C-terminus domain
Structure domains: BRCT domain
Histone H2AX Chains: I, J, K, L, M, N, O, P
Molecule details ›
Chains: I, J, K, L, M, N, O, P
Length: 10 amino acids
Theoretical weight: 1.24 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P16104 (Residues: 134-143; Coverage: 7%)
Gene names: H2AFX, H2AX

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 67.81Å b: 82.23Å c: 104.42Å
α: 72.1° β: 89.87° γ: 80.94°
R-values:
R R work R free
0.202 0.201 0.252
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided