› 3swd

X-ray diffraction
2.5Å resolution

E. coli MurA in complex with UDP-N-acetylmuramic acid and covalent adduct of PEP with Cys115

Released:
DOI: 10.2210/pdb3swd/pdb
PDB entry 3swd coloured by chain, front view.
PDB entry 3swd coloured by chain, side view.
PDB entry 3swd coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA).
Zhu JY, Yang Y, Han H, Betzi S, Olesen SH, Marsilio F, Schönbrunn E
J Biol Chem 287 12657-67 (2012)
PMID: 22378791

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-141532 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 418 amino acids
Theoretical weight: 44.91 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A749 (Residues: 1-418; Coverage: 100%)
Gene names: JW3156, b3189, murA, murZ
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

1 bound ligand:
Ligand EPZ 12 x EPZ
2 modified residues:
Ligand IAS 12 x IAS
Ligand QPA 12 x QPA

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 84.51Å b: 120.91Å c: 139.73Å
α: 111.52° β: 104.44° γ: 90.19°
R-values:
R R work R free
0.226 0.226 0.28
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Fosfomycin: Mechanism and Resistance.
Silver LL. (2017)
4 more

3 mentions without citation

Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA).
Zhu et al. (2012)
2 more