X-ray diffraction
3.05Å resolution

GLP (G9a-like protein) SET domain in complex with Dnmt3aK44me0 peptide


Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone-lysine N-methyltransferase EHMT1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 32.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q9H9B1 (Residues: 982-1266; Coverage: 22%)
Gene names: EHMT1, EUHMTASE1, GLP, KIAA1876, KMT1D
Sequence domains:
Structure domains: SET domain
DNA (cytosine-5)-methyltransferase 3A Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 12 amino acids
Theoretical weight: 1.26 KDa
Source organism: Mus musculus
Expression system: Not provided
  • Canonical: O88508 (Residues: 39-50; Coverage: 1%)
Gene name: Dnmt3a

Ligands and Environments

Cofactor: Ligand SFG 2 x SFG
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 80.14Å b: 91.14Å c: 95.07Å
α: 90° β: 90° γ: 90°
R R work R free
0.176 0.173 0.248
Expression systems:
  • Escherichia coli
  • Not provided