› 3su8

X-ray diffraction
3.2Å resolution

Crystal structure of a truncated intracellular domain of Plexin-B1 in complex with Rac1

Released:
DOI: 10.2210/pdb3su8/pdb
PDB entry 3su8 coloured by chain, front view.
PDB entry 3su8 coloured by chain, side view.
PDB entry 3su8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A dual binding mode for RhoGTPases in plexin signalling.
Bell CH, Aricescu AR, Jones EY, Siebold C
PLoS Biol 9 e1001134 (2011)
PMID: 21912513

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128844 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related C3 botulinum toxin substrate 1 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63000 (Residues: 1-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Plexin-B1 Chain: X
Molecule details ›
Chain: X
Length: 611 amino acids
Theoretical weight: 69.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O43157 (Residues: 1533-2135; Coverage: 29%)
Gene names: KIAA0407, PLXN5, PLXNB1, SEP
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 183.45Å b: 63.81Å c: 84.55Å
α: 90° β: 107.53° γ: 90°
R-values:
R R work R free
0.208 0.207 0.238
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

11 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
10 more

6 mentions without citation

Structural basis for activation and non-canonical catalysis of the Rap GTPase activating protein domain of plexin.
Wang et al. (2013)
5 more