› 3ss1

X-ray diffraction
2.2Å resolution

Clostridium difficile toxin A (TcdA) glucolsyltransferase domain

Released:
DOI: 10.2210/pdb3ss1/pdb
PDB 3ss1 coloured by chain and viewed from the front.
PDB 3ss1 coloured by chain and viewed from the side.
PDB 3ss1 coloured by chain and viewed from the top.
Source organism: Clostridioides difficile 630
Primary publication:
Structural determinants of Clostridium difficile toxin A glucosyltransferase activity.
Pruitt RN, Chumbler NM, Rutherford SA, Farrow MA, Friedman DB, Spiller B, Lacy DB
J. Biol. Chem. 287 8013-20 (2012)
PMID: 22267739

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosyltransferase TcdA Chain: A
Molecule details ›
Chain: A
Length: 555 amino acids
Theoretical weight: 64.4 KDa
Source organism: Clostridioides difficile 630
Expression system: Priestia megaterium
UniProt:
  • Canonical: P16154 (Residues: 1-542; Coverage: 20%)
Gene names: tcdA, toxA
Sequence domains:
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

1 bound ligand:
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P65
Unit cell:
a: 141.908Å b: 141.908Å c: 65.946Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.227
Expression system: Priestia megaterium

Quick links

Citations

15 review citations

Large Clostridial Toxins: Mechanisms and Roles in Disease.
Orrell et al. (2021)
14 more

9 mentions without citation

Clostridium difficile Toxins A and B: Insights into Pathogenic Properties and Extraintestinal Effects.
Di Bella et al. (2016)
8 more