3sq5

X-ray diffraction
2.3Å resolution

Crystal Structure Analysis of the Yeast Tyrosyl-DNA Phosphodiesterase H432N Mutant

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosyl-DNA phosphodiesterase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 470 amino acids
Theoretical weight: 54.03 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38319 (Residues: 79-539; Coverage: 85%)
Gene names: TDP1, YBR1520, YBR223C
Sequence domains: Tyrosyl-DNA phosphodiesterase
Structure domains: Endonuclease Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P1
Unit cell:
a: 64.453Å b: 82.036Å c: 98.677Å
α: 89.8° β: 94.33° γ: 112.78°
R-values:
R R work R free
0.204 0.203 0.236
Expression system: Escherichia coli BL21(DE3)