PDBe 3spy

X-ray diffraction
2.14Å resolution

RB69 DNA Polymerase(L415A/L561A/S565G/Y567A) Ternary Complex with dUpCpp Opposite dA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-directed DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 901 amino acids
Theoretical weight: 104.19 KDa
Source organism: Enterobacteria phage RB69
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38087 (Residues: 1-901; Coverage: 100%)
Gene name: 43
Sequence domains:
Structure domains:
5'-D(*TP*CP*GP*AP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3' Chain: T
Molecule details ›
Chain: T
Length: 18 nucleotides
Theoretical weight: 5.54 KDa
Source organism: Enterobacteria phage RB69
Expression system: Not provided
5'-D(*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*C)-3' Chain: P
Molecule details ›
Chain: P
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: Enterobacteria phage RB69
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 75.336Å b: 120.598Å c: 130.812Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.242
Expression systems:
  • Escherichia coli
  • Not provided