X-ray diffraction
3.18Å resolution

Methionine-adenosyltransferase from Entamoeba histolytica

Source organism: Entamoeba histolytica
Entry authors: Merritt EA, Medical Structural Genomics of Pathogenic Protozoa (MSGPP)

Function and Biology Details

Reaction catalysed:
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
S-adenosylmethionine synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 415 amino acids
Theoretical weight: 45.68 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
  • Canonical: C4M272 (Residues: 4-397; Coverage: 99%)
Gene name: EHI_174250
Sequence domains:
Structure domains: GMP Synthetase; Chain A, domain 3

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 58.492Å b: 113.161Å c: 220.848Å
α: 90° β: 90° γ: 90°
R R work R free
0.231 0.228 0.284
Expression system: Escherichia coli