PDBe 3si5

X-ray diffraction
2.2Å resolution

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 176 amino acids
Theoretical weight: 20.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60566 (Residues: 57-220; Coverage: 16%)
Gene names: BUB1B, BUBR1, MAD3L, SSK1
Sequence domains: Mad3/BUB1 homology region 1
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Kinetochore scaffold 1 Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 24 amino acids
Theoretical weight: 2.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NG31 (Residues: 234-252; Coverage: 1%)
Gene names: CASC5, KIAA1570, KNL1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR-H
Spacegroup: C2
Unit cell:
a: 124.398Å b: 40.143Å c: 75.391Å
α: 90° β: 91.38° γ: 90°
R-values:
R R work R free
0.196 0.193 0.247
Expression system: Escherichia coli