3sat

X-ray diffraction
2.15Å resolution

MUTM Slanted complex 6 with R112A mutation

Released:
Primary publication:
Strandwise translocation of a DNA glycosylase on undamaged DNA.
Proc. Natl. Acad. Sci. U.S.A. 109 1086-91 (2012)
PMID: 22219368

Function and Biology Details

Reactions catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Formamidopyrimidine-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 30.5 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84131 (Residues: 2-274; Coverage: 100%)
Gene names: fpg, mutM
Sequence domains: Zinc finger found in FPG and IleRS
Structure domains:
5'-D(*AP*GP*G*TP*AP*GP*AP*CP*CP*AP*GP*GP*AP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.96 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
5'-D(*TP*GP*CP*GP*T*CP*CP*TP*GP*GP*(TX2) P*CP*TP*AP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 45.487Å b: 97.574Å c: 102.371Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.225
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided