› 3s28

X-ray diffraction
2.8Å resolution

The crystal structure of sucrose synthase-1 in complex with a breakdown product of the UDP-glucose

Released:
DOI: 10.2210/pdb3s28/pdb
PDB 3s28 coloured by chain and viewed from the front.
PDB 3s28 coloured by chain and viewed from the side.
PDB 3s28 coloured by chain and viewed from the top.
Source organism: Arabidopsis thaliana
Primary publication:
The structure of sucrose synthase-1 from Arabidopsis thaliana and its functional implications.
Zheng Y, Anderson S, Zhang Y, Garavito RM
J. Biol. Chem. 286 36108-18 (2011)
PMID: 21865170

Function and Biology Details

Reaction catalysed: 
NDP-glucose + D-fructose = NDP + sucrose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sucrose synthase 1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 816 amino acids
Theoretical weight: 94.18 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49040 (Residues: 1-808; Coverage: 100%)
Gene names: At5g20830, SUS1, T1M15.230
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand UDP 8 x UDP
Ligand LCN 8 x LCN
Ligand NHF 8 x NHF
Ligand SO4 24 x SO4
Ligand MLA 8 x MLA
Ligand K 8 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 276.75Å b: 261.88Å c: 160.19Å
α: 90° β: 108.7° γ: 90°
R-values:
R R work R free
0.19 0.188 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

An Overview of Sucrose Synthases in Plants.
Stein et al. (2019)
6 more

5 mentions without citation

Identification of UDP-glucose binding site in glycosyltransferase domain of sucrose phosphate synthase from sugarcane (Saccharum officinarum) by structure-based site-directed mutagenesis.
Sawitri et al. (2018)
4 more