3rt0

X-ray diffraction
2.11Å resolution

Crystal structure of PYL10-HAB1 complex in the absence of abscisic acid (ABA)

Released:

Function and Biology Details

Reaction catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein phosphatase 2C 16 Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 37.75 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9CAJ0 (Residues: 172-511; Coverage: 70%)
Gene names: At1g72770, F28P22.4, HAB1, P2C-HA
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain
Abscisic acid receptor PYL10 Chains: C, D
Molecule details ›
Chains: C, D
Length: 183 amino acids
Theoretical weight: 20.66 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8H1R0 (Residues: 1-183; Coverage: 100%)
Gene names: At4g27920, PYL10, RCAR4, T13J8.30
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 70.766Å b: 83.503Å c: 88.544Å
α: 90° β: 97.2° γ: 90°
R-values:
R R work R free
0.175 0.173 0.207
Expression system: Escherichia coli